Chip-chip from wt and ino80 mutant cells with Htz1

ABSTRACT: Global regulation of H2A.Z localization by the INO80 chromatin remodeling enzyme is essential for genome integrity. Chromatin immunoprecipitation (ChIP) of Htz1 in wild-type and ino80 mutant yeast demonstrated that Ino80 plays a role in replacing Htz1 with H2A. Comparison of Htz1 localization in wt vs ino80 mutant yeast Prior to immunoprecipitation, cells arrested with alpha-factor or nocodazole: - The alpha-factor mating pheromone arrests yeast of the a mating type in the G1 phase of the cell cycle. - Nocodazole disrupts the polymerization of microtubules, blocking the cells from entering mitosis and arresting them in the G2/M phase of the cell cycle.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Oliver Rando 

PROVIDER: E-GEOD-25722 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress