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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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SAGE analysis of human glomerular endothelial cell

ABSTRACT: Tissue from macroscopically healthy parts of nephrectomized kidneys. Endothelial cells were purified from the cell outgrowth of glomeruli twice using CD31 magnetic beads and cultured on fibronectin coated surface. Cells were not passaged more than eight times and stained with vWf, CD31, and after stimulation with TNF alpha also with CD62E/P. This GEO Series was created by the GEO staff as part of a cleanup effort to ensure that all GEO Samples are included within a Series entry.

ORGANISM(S): Homo sapiens

SUBMITTER: Gürkan Sengoelge

PROVIDER: E-GEOD-25760 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SAGE analysis of human glomerular endothelial cell

Project description:Tissue from macroscopically healthy parts of nephrectomized kidneys. Endothelial cells were purified from the cell outgrowth of glomeruli twice using CD31 magnetic beads and cultured on fibronectin coated surface. Cells were not passaged more than eight times and stained with vWf, CD31, and after stimulation with TNF alpha also with CD62E/P.

2010-12-01 | GSE25760 | GEO

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SAGE analysis of maize root segments

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2010-12-01 | E-GEOD-25748 | biostudies-arrayexpress

Mouse_SAGE_P23_cerebellum

Project description:P23 C57BL6/J mouse cerebellum This GEO Series was created by the GEO staff as part of a cleanup effort to ensure that all GEO Samples are included within a Series entry.

2010-12-01 | E-GEOD-25751 | biostudies-arrayexpress

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Project description:SAGE libraries were prepared from mRNA of cultured bovine glomerular and aortic endothelial cells. Cells both cell lines were positive for acetylated LDL uptake and vWF expression, and were proven to be free of non-endothelial cells. Cultures were studied at passage 9-10. The cells were confluent, cultured in parallel, under identical conditions. Cells were plated on gelatin coated cell culture plastic in RPMI 1640 medium containing 10% FBS and streptomycin/penicillin. The last medium change was 48 hours prior to mRNA harvest. Keywords = endothelial, glomerular, kidney, aorta, Keywords: other

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