Gene expression changes in NFAT5/TonEBP under isotonic and hypertonic conditions

ABSTRACT: TonEBP is a transcription factor that promotes cellular accumulation of organic osmolytes in the hypertonic renal medulla by stimulating expression of its target genes. Genetically modified animals with deficient TonEBP activity in the kidney suffer from severe medullary atrophy in association with cell death, demonstrating that TonEBP is essential for the survival of the renal medullary cells. Using both TonEBP knockout cells and RNA interference of TonEBP, we found that TonEBP promoted cellular adaptation to hypertonic stress. Microarray analyses revealed that genetic response to hypertonicity was dominated by TonEBP in that expression of totally different sets of genes was increased by hypertonicity in those cells with TonEBP vs. those without TonEBP activity. Out of over 100 potentiall

ORGANISM(S): Mus musculus

SUBMITTER: Todd Lamitina 

PROVIDER: E-GEOD-25816 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress