Transcription profiling of S. cerevisiae IFO 0233 diploid strain sampled at multiple time point to investigate genome wide oscillations

ABSTRACT: In order to test the idea that the cell is an attractor and to examine in detail the connectivity relationships among genes, it was essential to have a very reproducible and precise biological system. Oscillations in respiratory activity in budding yeast and other organisms have been known for many years. This benchmark oscillation is now shown to be a global manifestation of a genome wide oscillation in transcription. Using Affymetrix S98 chips, the patterns of gene expression were followed by sampling at 4-minute intervals through three cycles of the DO oscillation. Phase assignments were made based on the dissolved oxygen cycle. Two different cultures were sampled and a total of 68 RNA samples were prepared. Choices of samples for microarray analysis were based on RNA quality, consist

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Robert Klevecz 

PROVIDER: E-GEOD-2583 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress