Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression patterns induced by 5-Fluorouracil in breast cancer cell lines

ABSTRACT: We have compared the changes in cell cycle and cell death parameters induced by 2 different concentrations of 5-FU (IC50 and IC80) in the breast adenocarcinoma cell line MCF7. G1/S cell cycle arrest was associated with both concentrations, whereas cell death was mainly induced after IC80 5-FU. These changes were correlated with gene expression assessed by cDNA microarray analysis. Main findings included an overexpression of p53 target genes involved in cell cycle and apoptosis (CDKN1A/p21, TP53INP, TNFRSF6/FAS and BBC3/PUMA), and significant repression of Myc. High dose 5-FU also induced a higher regulation of the mitochondrial death genes APAF1, BAK1 and BCL2, and induction of genes of the ID family. Time and dose-dependent changes in gene expression, induced by 5-Fluorouracil, in breast carcinoma cell lines MCF7, and MDA-MB-435.

ORGANISM(S): Homo sapiens

SUBMITTER: Gema Moreno-Bueno

PROVIDER: E-GEOD-2584 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional profiling of MCF7 breast cancer cells in response to 5-Fluorouracil: relationship with cell cycle changes and apoptosis, and identification of novel targets of p53.

Hernández-Vargas Héctor H Ballestar Esteban E Carmona-Saez Pedro P von Kobbe Cayetano C Bañón-Rodríguez Inmaculada I Esteller Manel M Moreno-Bueno Gema G Palacios José J

International journal of cancer 20060901 5

The availability of oral precursors of 5-Fluorouracil (5-FU) and its favorable results in treating advanced breast cancer have renewed the interest in the molecular mechanisms underlying its cytotoxicity. We have compared the changes in cell cycle and cell death parameters induced by 2 different concentrations of 5-FU (IC50 and IC80) in the breast adenocarcinoma cell line MCF7. G1/S cell cycle arrest was associated with both concentrations, whereas cell death was mainly induced after IC80 5-FU. ...[more]

PMID: 16557594

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Dataset Information

Gene expression patterns induced by 5-Fluorouracil in breast cancer cell lines

Publications

Transcriptional profiling of MCF7 breast cancer cells in response to 5-Fluorouracil: relationship with cell cycle changes and apoptosis, and identification of novel targets of p53.

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