Identification of genes upregulated by methyl-CpG targeted transcriptional activation (MeTA) in 293T

ABSTRACT: The identification of genes transcriptionally silenced by DNA hypermethylation is important in understanding the molecular basis of epigenetically regulated biological processes such as X chromosome inactivation, genomic imprinting, and cancer development. Our previously developed methyl-CpG targeted transcriptional activation (MeTA) method reactivates epigenetically silenced genes by using a methyl-CpG binding domain from MBD2 with a transcriptional activation domain. We applied either MeTA or a conventional DNA demethylating agent, 5-aza-cytidine (Aza-CR), to a human embryonic kidney cell line 293T and analyzed gene expression profiles by microarray; 138 and 202 genes that are upregulated 5-fold or more were identified by MeTA and Aza-CR, respectively. The top ten upregulated genes detec

ORGANISM(S): Homo sapiens

SUBMITTER: Shinichi Fukushige 

PROVIDER: E-GEOD-25981 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress