Aberrant brain miRNA target and miRISC gene expression in the anx/anx anorexia mouse model

ABSTRACT: The anorexia mouse model, anx/anx, carries a spontaneous mutation not yet identified and homozygous mutants are characterized by anorexia-cachexia, growth failure and abnormal behavior, such as head weaving, hyperactivity, and ataxia. In order to test if miRNA machinery could influence the phenotype of these mice, RNA samples from mutant and wild type hypothalamus and cortex were hybridized to microarray chips based on LNA probes allowing the detection of 279 mouse miRNAs and to Agilent 44k chips to analyze the whole mRNA transcriptome. In anx/anx hypothalamus we found that predicted miRNA targets were preferentially upregulated in a lineally dependent manner according to the number of miRNA target sites in each mRNA (p = 10-139). Conversely, we observed that in anx/anx cortex mRNAs pred

ORGANISM(S): Mus musculus

SUBMITTER: Lauro Sumoy 

PROVIDER: E-GEOD-26003 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress