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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Regulation of transcription by HIP1 protein interactor (HIPPI) in HeLa cells

ABSTRACT: HIPPI is a novel transcription factor that regulates caspase1 gene expression. To find out new transcriptional targets of HIPPI and to investigate the effect of HIPPI on cellular transcriptome, microarray study is performed using HeLa cells exogenously transfected with Green Fluorescent Protein tagged HIPPI (GFP-Hippi) construct Total RNA extracted from HeLa cells transfected with empty GFP vector served as control and Total RNA extracted from HeLa cells transfected with GFP-Hippi construct served as test. Biological replicates: 4

ORGANISM(S): Homo sapiens

SUBMITTER: Nitai Bhattacharyya

PROVIDER: E-GEOD-26115 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Role of HIP1 in HIPPI mediated transcription regulation in HeLa cells

Project description:Nuclear entry of transcription factor HIPPI is mediated by its interacting partner Huntingtin Interacting Protein 1 (HIP1), a nuclear localization signal containing nucleo-cytoplasmic shuttling protein. In oredr to investigate the role of HIP1 in HIPPI mediated transcriptional regulation in cell, here we performed microarray experiments using stable HIP1 knocked down HeLa cells (Hip1Si) exogenously expressing Green fluorescent protein tagged HIPPI. Total RNA extracted from HIP1 knocked down HeLa cells (Hip1Si) transfected with empty GFP vector served as control and Total RNA extracted from Hip1Si cells transfected with GFP-Hippi construct served as test. Biological replicates: 4

2011-09-20 | E-GEOD-26116 | biostudies-arrayexpress

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Regulation of transcription by HIP1 protein interactor (HIPPI) in HeLa cells

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