Assessment of genome integrity with array CGH of cattle transgenic cell lines produced by homologous recombination and somatic cell cloning

ABSTRACT: Background Transgenic cattle carrying multiple genomic modifications have been produced by sequential gene targeting and serial rounds of somatic cell chromatin transfer (cloning). However, cloning efficiency tends to decline with the increase of rounds of cloning. It is possible that multiple rounds of cloning compromise the genome integrity, rendering a decline in cloning. To test this possibility, we performed 9 high density array Comparative Genomic Hybridization (CGH) experiments to test the genome integrity in 3 independent bovine transgenic cell lineages generated from serial rounds of genetic modification and cloning. Our plan included the control hybridizations (self to self) of 3 founder cell lines and 6 comparative hybridizations between these founders and their derived cell lin

ORGANISM(S): Bos taurus

SUBMITTER: George Liu 

PROVIDER: E-GEOD-26132 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress