Regulation of DNA methylation by CK2-mediated phosphorylation of DNMT3A (Beadchip)

ABSTRACT: DNA methylation is a central epigenetic modification that has essential roles in cellular processes including chromatin structure, gene regulation, development and disease. The de novo DNA methyltransferases are responsible for the generation of genomic methylation patterns, but the underlying mechanisms are still poorly understood. Here, we show that phosphorylation of DNMT3A by the CK2 protein kinase regulates the establishment of DNA methylation patterns. We find that DNMT3A is phosphorylated by CK2 at two key residues located near its PWWP domain. We observed that, through phosphorylation of these residues, CK2 negatively regulates DNMT3AM-bM-^@M-^Ys ability to methylate DNA and consistent with this, CK2 was found to decrease overall genomic level of 5-methylcytosine. Further, genome-w

ORGANISM(S): Homo sapiens

SUBMITTER: Matthieu Defrance 

PROVIDER: E-GEOD-26164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress