Lieb SDQ4051_LEM2_N2_MXEMB
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ABSTRACT: modENCODE_submission_3154 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: To complement our extensive ChIP-chip characterization of chromosomal regulators in C. elegans, in select experiments we also isolate total RNA from parallel cultures of staged animals. Labeled cDNA will be prepared and used to probe either gene-specific microarrays, or genomic tiling microarrays to assess the transcriptional state of genomic sequences flanking the protein-binding regions localized by ChIP-chip and ChIP-seq. To investigate possible correlations between putative regulatory proteins and transcription state, data from such experiments (i.e., protein binding sites and steady-state mRNA levels) will be integrated during downstream analysis. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Mixed Embryo; Genotype: wild type; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain N2; Developmental Stage Mixed Embryo; temp (temperature) 20 degree celsius
ORGANISM(S): Caenorhabditis elegans
SUBMITTER: DCC modENCODE
PROVIDER: E-GEOD-26201 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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