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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genomic Library Enrichment for n-Butanol tolerance in E. coli. Samples vs Reference

ABSTRACT: Genomic Library Enrichment to determine the n-Butanol Tolerant related genes in E. coli. The samples involves a series of batch transfers to increasing concentrations of n-butanol and controls (always grew in absence of the solvent) Two technical replicas. Samples 1, 3, 5, and 7 are the serial transfers (samples 15, 13, 11, and 9 are their respective replicas). Samples 2, 4, 6 and 8 are the controls grown in absence of n-butanol (samples 16, 14, 12 and 10 are their respective replicas)

ORGANISM(S): Escherichia coli

SUBMITTER: James Winkler

PROVIDER: E-GEOD-26223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genomic library screens for genes involved in n-butanol tolerance in Escherichia coli.

Reyes Luis H LH Almario Maria P MP Kao Katy C KC

PloS one 20110308 3

<h4>Background</h4>n-Butanol is a promising emerging biofuel, and recent metabolic engineering efforts have demonstrated the use of several microbial hosts for its production. However, most organisms have very low tolerance to n-butanol (up to 2% (v/v)), limiting the economic viability of this biofuel. The rational engineering of more robust n-butanol production hosts relies upon understanding the mechanisms involved in tolerance. However, the existing knowledge of genes involved in n-butanol to ...[more]

PMID: 21408113

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Genomic Library Enrichment for n-Butanol tolerance in E. coli. Samples vs Reference

Project description:Genomic Library Enrichment to determine the n-Butanol Tolerant related genes in E. coli. The samples involves a series of batch transfers to increasing concentrations of n-butanol and controls (always grew in absence of the solvent)

2012-09-19 | GSE26223 | GEO

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Project description:Transcriptome analysis of isolated mutants using the method Visualizing Evolution in Real-Time (VERT) for the study of n-butanol tolerance. The samples were isolated from an evolution experiment picking samples at different times based in the evolution dynamics obtained with VERT. Mutants were grown in chemostats at 0.8% (v/v) of n-butanol and compared with the expression of wild-type to the same concentration of solvent. Three biological replicas. Each sample represents an isolated mutant. The reference for each mutant corresponds to wild-type, both exposed to the same concentration of n-butanol (0.8% (v/v)).

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2013-07-01 | GSE47589 | GEO

Butanol Challenge of L. brevis

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