MC70 potentiates doxorubicin efficacy in colon and breast cancer in vitro treatment

ABSTRACT: A major limitation in the cancer treatment is the ability of cancer cells to become resistant to chemotherapeutic drugs, by multidrug establishment. Here, we evaluate the possibility to utilize MC70, either as ABC transporters inhibitor or as anticancer agent, in monotherapy or in combination with doxorubicin for cancer treatment. The study was carried out in MCF7/ADR and Caco-2, breast and colon cancer cells, respectively. Cell growth and apoptosis were measured by MTT assay and DNA laddering Elisa kit, respectively. Cell cycle perturbation and cellular targets modulation were analyzed by flow cytometry and western blotting, respectively. MC70 was analyzed for its interaction with ABC transporters, MDR-1, BCRP and MRP-1, and for its anticancer activity. In MCF7/ADR, MC70 slight inhibite

ORGANISM(S): Homo sapiens

SUBMITTER: Pina Iannelli 

PROVIDER: E-GEOD-26250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress