A systematic spatial bias in microarray hybridizations caused by probe spot position-dependent variability in lateral diffusion

ABSTRACT: The hybridization of nucleic acid targets with surface-immobilized probes is a widely used assay format for high-throughput detection of many parallel targetsin medical and biological research. Though commonly applied, microarray technology still suffers limitations arising from problems of data robustness and reproducibility across platforms, stemming in part from an incomplete understanding of the complex processes governing surface hybridization behavior. It has been observed that there are non-random spatial variations within individual microarray hybridizations, but the causative mechanisms of positional bias remain largely unexplained. This study identifies a symptomatic spatial bias in surface hybridization signal intensitywith systematically increased signal intensities of spots lo

ORGANISM(S): Desulfovibrio halophilus

SUBMITTER: Doris Steger 

PROVIDER: E-GEOD-26275 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress