Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide assessment of imprinted expression in human cells

ABSTRACT: Parent-of-origin dependent expression of alleles, imprinting, has been suggested to impact a substantial proportion of mammalian genes. Its discovery requires allele-specific detection of expressed transcripts, but in some cases detected allelic expression (AE) bias has been interpreted as imprinting without demonstrating compatible transmission patterns and excluding heritable variation. Therefore, we utilized a genome-wide tool exploiting high density genotyping arrays in parallel measurements of genotypes in RNA and DNA to determine AE across the transcriptome in lymphoblastoid cell lines (LCLs) and skin fibroblasts derived from families. To investigate imprinting, transmission patterns were analyzed in 2 LCL trios, 1 LCL 3-generation pedigree and 9 FB trios. To investigate random monoallelic expression, 2 cell lines were treated with three concentrations of 5-azadeoxycytidine (AZA), and one cell line was treated for two time periods.

ORGANISM(S): Homo sapiens

SUBMITTER: Tomi Pastinen

PROVIDER: E-GEOD-26286 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide assessment of imprinted expression in human cells.

Morcos Lisanne L Ge Bing B Koka Vonda V Lam Kevin C L KC Pokholok Dmitry K DK Gunderson Kevin L KL Montpetit Alexandre A Verlaan Dominique J DJ Pastinen Tomi T

Genome biology 20110321 3

<h4>Background</h4>Parent-of-origin-dependent expression of alleles, imprinting, has been suggested to impact a substantial proportion of mammalian genes. Its discovery requires allele-specific detection of expressed transcripts, but in some cases detected allelic expression bias has been interpreted as imprinting without demonstrating compatible transmission patterns and excluding heritable variation. Therefore, we utilized a genome-wide tool exploiting high density genotyping arrays in paralle ...[more]

PMID: 21418647

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Dataset Information

Genome-wide assessment of imprinted expression in human cells

Publications

Genome-wide assessment of imprinted expression in human cells.

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