LIF-Dependent, Pluripotent Stem Cells Established from Inner Cell Mass of Porcine Embryos

ABSTRACT: The pig is important for agriculture and as an animal model in human and veterinary medicine, yet, despite over 20 years of effort, it has proved a difficult species from which to generate pluripotent stem cells analogous to those derived from mouse embryos. Here we report the production of LIF-dependent, so called naM-CM-/ve type, pluripotent stem cells from the inner cell mass of porcine blastocysts by up-regulating expression of KLF4 and POU5F1. These cells resemble mouse ES cells and are distinct from the FGF2-dependent, induced pluripotent cell type derived from porcine somatic cells. Transcript profiling utilized Affymetrix porcine microarrays were conducted to compare the gene expressions associated with pluripotency in the two LIF-dependent pESK lines (pESK I & II, passage 14 & 5,

ORGANISM(S): Sus scrofa

SUBMITTER: Toshihiko Ezashi 

PROVIDER: E-GEOD-26369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress