ABSTRACT: Genome-wide transcriptional profiling of Bj110 cells treated with P6 (20 uM for 24 h). Three independent biological materials were prepared for cells treated with P6 or DMSO (vehicle control). A total 6 arrays which includes dye swap were analyzed.
Project description:Transcriptional profiling of chemoautotrophically and arabinose supplemented chemoautotrophically grown cells Keywords: Comparison of different lifestyles Three independent biological materials were prepared for arabinose supplemented chemoautotrophically cultured cells and chemoautotrophically cultured cells. Total 6 arrays including dye swap were analyzed.
Project description:Transcriptional profiling of arabinose supplemented chemoautotrophically grown cells Keywords: Comparison of different lifestyles Three independent biological materials were prepared for arabinose supplemented chemoautotrophically cultured cells and heterotrophically cultured cells. Total 6 arrays including dye swap were analyzed.
Project description:Transcriptional profiling of chemoautotrophically grown cells Keywords: Comparison of different lifestyles Two independent biological materials were prepared for chemoautotrophically cultured cells and heterotrophically cultured cells. Total 4 arrays including dye swap were analyzed.
Project description:Genome-wide transcriptional profiling of cells subjected to a H2O2 shock treatment (10 mM for 10 minutes). Three independent biological materials were prepared for cells shock treated with H2O2 and non-treated cells. A total 6 arrays which includes dye swap were analyzed.
Project description:Genome-wide transcriptional profiling of cells subjected to a paraquat fulminant shock treatment (5mM for 10 minutes). Three independent biological materials were prepared for cells shock treated with paraquat and non-treated cells. A total 6 arrays which includes dye swap were analyzed.
Project description:Genome-wide transcriptional profiling of cells exposed to 0.3 mM H2O2 from time of inoculation. Three independent biological materials were prepared for cells exposed to 0.3 mM H2O2 during growth and non-treated cells. A total 6 arrays which includes dye swap were analyzed
Project description:Genome-wide transcriptional profiling of cells exposed to 0.1 mM paraquat from time of inoculation. Three independent biological materials were prepared for cells exposed to 0.1 mM paraquat during growth and non-treated cells. A total 6 arrays which includes dye swap were analyzed.
Project description:This SuperSeries is composed of the following subset Series: GSE10295: Bj_Heterotrophy vs. Arabinose supplemented chemoautotrophy GSE10296: Bj_Heterotrophy vs. Chemoautotrophy GSE10298: Bj_Chemoautotrophy vs. Arabinose supplemented chemoautotrophy Refer to individual Series
Project description:This SuperSeries is composed of the following subset Series: GSE26236: Bj Paraquat fulminant shock vs. non-treatment GSE26252: Bj Paraquat prolonged exposure vs. non-treatment Refer to individual Series
Project description:Elevated atmospheric CO2 can influence the structure and function of rhizosphere microorganisms by altering root growth and the quality and quantity of compounds released into the rhizosphere via root exudation. In these studies we investigated the transcriptional responses of Bradyrhizobium japonicum cells growing in the rhizosphere of soybean plants exposed to elevated atmospheric CO2. Transciptomic expression profiles indicated that genes involved in carbon/nitrogen metabolism, and FixK2-associated genes, including those involved in nitrogen fixation, microanaerobic respiration, respiratory nitrite reductase, and heme biosynthesis, were significantly up-regulated under conditions of elevated CO2, relative to plants and bacteria grown under ambient CO2 growth conditions. The expression profile of genes involved in lipochitinoligosaccharide Nod factor biosynthesis and negative transcriptional regulators of nodulation genes, nolA and nodD2, were also influenced by plant growth under conditions of elevated CO2. Taken together, results of these studies indicate that growth of soybeans under conditions of elevated atmospheric CO2 influences gene expressions in B. japonicum in the soybean rhizosphere, resulting in changes to carbon/nitrogen metabolism, respiration, and nodulation efficiency. Bradyrhizobium japonicum strains were grown in the soybean rhizosphere under two different CO2 concentrations. Transcriptional profiling of B. japonicum was compared between cells grown under elevated CO2 and ambient conditions. Four biological replicates of each treatment were prepared, and four microarray slides were used for each strain.