Comparison of two hepatocellular cancer cell lines with distinct properties regarding epithelial-mesenchymal transition

ABSTRACT: The epithelial to mesenchymal transition (EMT) of malignant hepatocytes is a crucial event in hepatocellular carcinoma (HCC) progression and recurrence. We aimed to establish a human model of EMT to examine drug efficacy and specificity in HCC progression. Human HCC cell populations were characterized by immunofluorescence analysis, migration and invasion assays, array comparative genomic hybridization, whole-genome expression profiling and promoter methylation. Therapeutic agents clinically used against HCC were examined for efficacy by determination of IC50 values. Liver cancer cell lines showed either an epithelial or mesenchymal phenotype of which latter showed strong migratory and invasive abilities in vitro. The common cellular origin of both cell types indicated that mesenchymal HCC

ORGANISM(S): Homo sapiens

SUBMITTER: Martin Bilban 

PROVIDER: E-GEOD-26391 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress