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Roles of RDR701 and DCLs in Rice PhasiRNA biogenesis

ABSTRACT: In this study, we analyzed function of the rice RDR701 and various DCLs proteins in small RNA processing. small RNA deep sequencing analyses revealed that RDR701 and OsDCL4 is involved in tasiRNAs and 21- and 24-nt phasiRNAs biogenesis. Further analyses indicate RDR701 coupling with OsDCL4 and OsDCL3b are required for the biogenesis of 21- and 24-nt phasiRNAs, respectively. The triggering miRNAs for intial both size phasiRNAs,are also shown RDR701- and OsDCL4-dependent, suggesting two sequential round activity of RDR701 in associated with different DCLs are essential for rice phasiRNAs biogenesis. Four small RNA libraries were constructed from panicles of zhonghua11 (a wild-type Japonica rice variety) and rdr701-1 at high temperature (Day average temperature 32M-bM-^DM-^C) and low temperature (Day average temperature 28M-bM-^DM-^C).

ORGANISM(S): Oryza sativa

SUBMITTER: xiaofeng cao

PROVIDER: E-GEOD-26405 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rice RNA-dependent RNA polymerase 6 acts in small RNA biogenesis and spikelet development.

Song Xianwei X Wang Dekai D Ma Lijia L Chen Zhiyu Z Li Pingchuan P Cui Xia X Liu Chunyan C Cao Shouyun S Chu Chengcai C Tao Yuezhi Y Cao Xiaofeng X

The Plant journal : for cell and molecular biology 20120605 3

Higher plants have evolved multiple RNA-dependent RNA polymerases (RDRs), which work with Dicer-like (DCL) proteins to produce different classes of small RNAs with specialized molecular functions. Here we report that OsRDR6, the rice (Oryza sativa L.) homolog of Arabidopsis RDR6, acts in the biogenesis of various types and sizes of small RNAs. We isolated a rice osrdr6-1 mutant, which was temperature sensitive and showed spikelet defects. This mutant displays reduced accumulation of tasiR-ARFs, ...[more]

PMID: 22443269

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