Deep sequencing-based analysis of the anaerobic stimulon in Neisseria gonorrhoeae

ABSTRACT: Maintenance of an anaerobic respiratory system in the obligate human pathogen, Neisseria gonorrhoeae, suggests that an anaerobic lifestyle may be important during the course of infection. However, at this point there have been no studies analyzing the complete gonococcal transcriptome response to anaerobiosis. Here we performed deep sequencing to compare the gonococcal transcriptomes of aerobic and anaerobically grown cells. We found that the anaerobic stimulon in gonococci was large, and that 198 chromosomal genes were found to be differentially expressed. We also observed a large induction of genes encoded within the cryptic plasmid, pJD1. Secondary analysis of genes found to be differentially expressed by RNA-seq using translational-lacZ fusions or RT-PCR demonstrated the RNA-seq re

ORGANISM(S): Neisseria gonorrhoeae F62

SUBMITTER: Vincent Isabella 

PROVIDER: E-GEOD-26444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress