Transcription profiling of human soluble guanylate cyclase-deficient U937 cells exposed to the nitric oxide (NO) donor S-nitrosoglutathione to provide insight into the cGMP-independent effects of NO

ABSTRACT: NO effects on gene expression, independent of cGMP, were examined globally. Differentiated human U937 cells that lack soluble guanylate cyclase were exposed to Snitrosoglutathione, a NO donor, or glutathione without or with dibutyryl-cAMP (Bt2cAMP). NO regulated 110 transcripts that annotated disproportionately to the cell cycle and cell proliferation (47/110, 43%) and more frequently than expected contained AU-rich, post-transcriptional regulatory elements (ARE). Bt2cAMP regulated 106 genes; cell cycle gene enrichment did not reach significance. Like NO, Bt2cAMP was associated with ARE-containing transcripts. Cell cycle genes induced by NO were G1/S phase associated (7/8) including E2F1 and p21/Waf1/Cip1; 6 of these 7 were E2F target genes involved in G1/S transition. Repressed genes were

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Danner 

PROVIDER: E-GEOD-2649 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress