Integration of molecular biology tools to obtain information about abundantly expressed genes in flowers of Oncidium Gower Ramsey

ABSTRACT: Orchids are commercially important flowers, comprising one of the largest families of plants. Model plants such as Arabidopsis do not contain all plant genes. In this study, several molecular biology tools were integrated to isolate the flower-specific gene promoters of Oncidium Gower Ramsey (GR). A cDNA library of reproductive tissues was constructed and a microarray was established and used to compare gene expression in flowers and leaves. Five genes were highly expressed in flower tissues and the subcellular locations of the corresponding proteins were identified using fluorescent protein-fusion protein lip transient transformation, and related to their putative functions. BAC clones of the five genes, together with nine previously published flower and reproductive growth specific genes

ORGANISM(S): Oncidium Gower Ramsey

SUBMITTER: Choun Sea Lin 

PROVIDER: E-GEOD-26504 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress