Two distinct auto-regulatory loops operate at the Pu.1 locus in B cells and myeloid cells

ABSTRACT: The transcription factor PU.1 occupies a central role in controlling myeloid and early B cell development and its correct lineage-specific expression is critical for the differentiation choice of hematopoietic progenitors. However, little is known of how this tissue-specific pattern is established. We previously identified an upstream regulatory cis-element (URE) whose targeted deletion in mice decreases PU.1 expression and causes leukemia. We show here that the URE alone is insufficient to confer physiological PU.1 expression, but requires the cooperation with other, previously unidentified elements. Using a combination of transgenic studies, global chromatin assays and detailed molecular analyses we present evidence that Pu.1 is regulated by a novel mechanism involving cross-talk between

ORGANISM(S): Mus musculus

SUBMITTER: Salam Assi 

PROVIDER: E-GEOD-26550 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress