Ubiquitous expression of long dsRNA in mice causes mainly RNAi effects in the oocyte

ABSTRACT: Double-stranded RNA (dsRNA) can enter different pathways in mammalian cells, such as the sequence-specific RNA interference (RNAi) pathway, the sequence-independent interferon response, and RNA editing by adenosine deaminases. To study routing of long dsRNA into different pathways in different tissues, we generated transgenic mice carrying an inverted repeat transcribed by a strong, ubiquitously active polII promoter. Here, we provide the first report of effects caused by ubiquitous long dsRNA expression in the adult mouse. Long dsRNA is poorly processed into siRNAs in somatic cells but readily induces the RNAi effects in the oocyte, suggesting that somatic cells are missing a component of RNAi that facilitates siRNA biogenesis. Expression of dsRNA is not sufficient to activate the interfe

ORGANISM(S): Homo sapiens

SUBMITTER: Radek Malik 

PROVIDER: E-GEOD-26577 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress