Genes for embryo development are packaged in blocks of multivalent chromatin in zebrafish sperm

ABSTRACT: In mature human sperm, genes of importance for embryo development (i.e. transcription factors) lack DNA methylation and bear nucleosomes with distinctive histone modifications, suggesting the specialized packaging of these developmental genes in the germline. Here, we explored the tractable zebrafish model and found conceptual conservation as well as several new features. Biochemical and mass spectrometric approaches reveal the zebrafish sperm genome packaged in nucleosomes and histone variants (and not protamine), and we find linker histones high and H4K16ac absent - key factors which may contribute to genome condensation. We examined several activating (H3K4me2/3, H3K14ac, H2AFV) and repressing (H3K27me3, H3K36me3, H3K9me3, hypoacetylation) modifications/compositions genome-wide, and fin

ORGANISM(S): Danio rerio

SUBMITTER: Shan-Fu Wu 

PROVIDER: E-GEOD-26609 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress