Molecular Mapping of Keratinocyte Differentiation by Functional Gene Expression Profiling

ABSTRACT: Our goal was to establish a robust and reliable platform for the mapping of gene expression changes occurring during the process of keratinocyte differentiation. We hypothesized that a functional gene expression profiling approach capable of identifying the program of gene expression over time would facilitate more focused identification of relevant genes. To achieve this, we used the confluenceinduced differentiation (CID) model, a widely used and reliable method that in many aspects recapitulates in vivo differentiation of human epidermal keratinocytes (Paragh et al, 2010; Poumay and Pittelkow, 1995). Primary human keratinocytes from three donors were subjected to CID in separate cultures and harvested at successive time points including 2 days prior to reaching confluence (D(-2)), at th

ORGANISM(S): Homo sapiens

SUBMITTER: Yoga Balagurunathan 

PROVIDER: E-GEOD-26688 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress