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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Zebrafish 27hpf embryos: hdac1 mutant (hi1618) vs sibling

ABSTRACT: Transcriptional profiling of hdac1 mutant zebrafish in comparison to their sibling embryos. Embryos resulting from a cross between heterozygous hdac1 mutant zebrafish (hi1618/+) where cultured together then mutants separated from the siblings one the basis of phenotype and RNA extracted from the two groups at 27hpf was compared in a two-colour hybridisation. Two-condition experiment, hdac1 mutants vs. sibling. Biological replicates: 2 (separate mating) Technical replicates: 4 (2 of which are dye-swap)

ORGANISM(S): Danio rerio

SUBMITTER: Michael Harrison

PROVIDER: E-GEOD-26707 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The epigenetic regulator Histone Deacetylase 1 promotes transcription of a core neurogenic programme in zebrafish embryos.

Harrison Michael R M MR Georgiou Aristophanes S AS Spaink Herman P HP Cunliffe Vincent T VT

BMC genomics 20110112

<h4>Background</h4>The epigenetic regulator Histone Deacetylase 1 (Hdac1) is required for specification and patterning of neurones and myelinating glia during development of the vertebrate central nervous system (CNS). This co-ordinating function for Hdac1 is evolutionarily conserved in zebrafish and mouse, but the mechanism of action of Hdac1 in the developing CNS is not well-understood.<h4>Results</h4>A genome-wide comparative analysis of the transcriptomes of Hdac1-deficient and wild-type zeb ...[more]

PMID: 21226904

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