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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The Nucleosome Map of the Mammalian Liver

ABSTRACT: We report the genome-wide map of nucleosome positions in the mouse liver, with emphasis on transcriptional start sites, CpG islands, Foxa2 binding sites, and their correlation with gene expression. Despite the heterogeneity of liver tissue, we could clearly discern the nucleosome pattern of the predominant liver cell, the hepatocyte. By analyzing nucleosome occupancy and the distributions of heterochromatin protein 1 (Hp1), CBP, and p300 in Foxa1/2-deficient livers we find, surprisingly, that the maintenance of nucleosome position and chromatin structure surrounding Foxa2 binding sites is independent of Foxa1/2. Examination of nucleosome map and Foxa2 binding in the mouse liver.

ORGANISM(S): Mus musculus

SUBMITTER: Jonathan Schug

PROVIDER: E-GEOD-26729 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The nucleosome map of the mammalian liver.

Li Zhaoyu Z Schug Jonathan J Tuteja Geetu G White Peter P Kaestner Klaus H KH

Nature structural & molecular biology 20110529 6

Binding to nucleosomal DNA is critical for 'pioneer' transcription factors such as the winged-helix transcription factors Foxa1 and Foxa2 to regulate chromatin structure and gene activation. Here we report the genome-wide map of nucleosome positions in the mouse liver, with emphasis on transcriptional start sites, CpG islands, Foxa2 binding sites and their correlation with gene expression. Despite the heterogeneity of liver tissue, we could clearly discern the nucleosome pattern of the predomina ...[more]

PMID: 21623366

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