Analysis of a normalised equine cDNA library using deep sequencing

ABSTRACT: While a first draft of the equine genome is available and predictions are made regarding resulting genes and proteins, little is known about the actual transcriptome. So far, published expressed sequence tags (ESTs) from different horse tissues were generally rather short (?600bp) and hardly annotated, reflecting the problem that good cDNA libraries are very difficult to analyse. In this approach, we aimed to establish and analyse a normalised immune cell cDNA library (using freshly isolated and activated lymphocytes, NK cells, monocytes and DC). In particular, we wanted to test next generation sequencing combined with a series of bioinformatic approaches. The resulting cDNA library contained 2x107 clones of which 1056 were used for an initial Sanger sequencing and 4x106 for the deep seque

ORGANISM(S): Equus caballus

SUBMITTER: Brigitte Waegele 

PROVIDER: E-GEOD-26794 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress