Isolation of epiblast stem cells from pre-implantation mouse embryos

ABSTRACT: Pluripotent stem cells provide a platform to interrogate control elements that function to generate all cell types of the body. Despite their utility for modeling development and disease, the relationship of mouse and human pluripotent stem cell states to one another remains largely undefined. We have shown that mouse embryonic stem (ES) cells and epiblast stem cells (EpiSCs) are distinct, pluripotent states isolated from pre- and post-implantation embryos respectively. Human ES cells are different than mouse ES cells and share defining features with EpiSCs, yet are derived from pre-implantation human embryos. Here we show that EpiSCs can be routinely derived from pre-implantation mouse embryos. The pre-implantation-derived EpiSCs exhibit molecular features and functional properties co

ORGANISM(S): Mus musculus

SUBMITTER: Paul Tesar 

PROVIDER: E-GEOD-26814 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress