Targeted bisulfite sequencing by solution hybrid selection and massively parallel sequencing

ABSTRACT: We applied the solution hybrid selection approach to the enrichment of CpG islands (CGIs) and promoter sequences from the human genome for targeted high-throughput bisulfite sequencing. A single lane of Illumina sequences allowed accurate and quantitative analysis of 1 million CpGs in more than 21,408 CGIs and 15,946 transcriptional regulatory regions. More than 85% of capture probes successfully yielded quantitative DNA methylation information of targeted regions. In this study, we generated genome-wide, single-base resolution DNA methylation maps in three of the most commonly used breast cancer cell lines.Differentially methylated regions (DMRs) were identified in the 5?-end regulatory regions, as well as the intra- and intergenic regions, particularly in the X chromosome among the thre

ORGANISM(S): Homo sapiens

SUBMITTER: Huidong Shi 

PROVIDER: E-GEOD-26826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress