Gene expression profile of H1.5 knockdown and SIRT1 knockdown IMR90 cells versus control cells

ABSTRACT: In humans, there are eleven subtypes of linker histones that exhibit cell- and tissue-specific expression. Linker histone H1 proteins bind to both the core histones and linker DNA of chromatin fibers; and not only participate in control of gene activity but also serve to stabilize higher order chromatin structure. To determine the potential roles of linker histones in differentiation, we examined the global distribution of linker histone subtype H1.5 in human IMR90 fibroblasts and H1 embryonic stem cells (hESCs). Surprisingly, H1.5 binds to and represses a large fraction of gene family clusters in fully differentiated cell types representing all three embryonic germ layers. Little or no H1.5 enrichment at gene family clusters was detected in undifferentiated hESCs or partially differentiat

ORGANISM(S): Homo sapiens

SUBMITTER: Jing-Yu Li 

PROVIDER: E-GEOD-26856 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress