Expression data of human eGFP-SOX17+ endoderm cells

ABSTRACT: Using homologous recombination in human ESC, we inserted an enhanced green fluorescent protein (eGFP) transgene into a locus encoding a postulated marker of human endoderm, SOX17 in H9 human embryonic stem cells. This allowed purification of SOX17+ hESC endodermal progeny by fluorescence activated cell sorting (FACS) to generate microarray gene expression profile. Using Wnt3 and Activin to differentiate hSOX17-2 to stage 1 cells, and subsequently FGF10 and cyclopamine to stage 2 cells, we isolated eGFP+ cells by FACS at each stage, performed microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Pei Wang 

PROVIDER: E-GEOD-26862 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress