Gene expression changes induced by Benzo[a]pyrene (BaP) in human breast carcinoma cell line MCF-7 in different phases of the cell cycle

ABSTRACT: Benzo[a]pyrene (BaP) is a widespread environmental genotoxic carcinogen that damages DNA by forming adducts. This damage with along the activation of the aryl hydrocarbon receptor (AHR) induces complex transcriptional responses in cells. To investigate whether human cells are more susceptible to BaP in a particular phase of the cell cycle, synchronised breast carcinoma MCF-7 cells were exposed to BaP for 12 h. Agilent Whole Human 44K Genome Oligo Arrays and RT-PCR were used to detect gene expression (mRNA) changes. Genes that were found to have altered expression included those involved in xenobiotic metabolism, apoptosis, cell cycle regulation and DNA repair. Gene ontology and pathway analysis showed the involvement of various signalling pathways in response to BaP exposure, such as the C

ORGANISM(S): Homo sapiens

SUBMITTER: Ian Giddings 

PROVIDER: E-GEOD-26917 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress