PhyK, PhyR, σT and σU regulons

ABSTRACT: The extracytoplasmic function sigma factor σT is the master regulator of general stress response in Caulobacter crescentus, and controls the expression of its paralogue σU. This work showed that PhyR and NepR act as positive and negative regulators, respectively, of σT expression and function. Biochemical data also indicated that NepR directly binds σT and the phosphorylated form of PhyR. We also demonstrated the essential role of the histidine kinase gene CC3474, here denominated phyK, for expression of σT-dependent genes and for resistance to stress conditions. Additionally, in vivo evidence of PhyK-dependent phosphorylation of PhyR is presented. This study also identified a conserved cysteine residue (C95) present in the periplasmic portion of PhyK that is crucial for the function of th

ORGANISM(S): Caulobacter crescentus NA1000

SUBMITTER: Rogerio Lourenco 

PROVIDER: E-GEOD-26977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress