Responses to ectoparasite salmon louse (Lepeophtheirus salmonis) in Atlantic salmon
Ontology highlight
ABSTRACT:
ORGANISM(S): Salmo salar
SUBMITTER: Aleksei Krasnov
PROVIDER: E-GEOD-27013 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:Gene expression profiling revealed rapid activation of immunity, both local and systemic, which however did not provide protection of fish against the parasite. Major changes of transcriptome responses wwere observed between days 5 and 10 Atlantic salmon was challenged with L. salmonis at the copepod stage. Skin, spleen, and head kidney were sampled from challenged and control fish at 1, 3, 5 dpi (corresponding to the copepod stage); 10 and 15 dpi (chalimus stage). A total of forty samples of spleen and skin form infected salmon (4 individuals from the 5 time points) were used for microarray analyses.. Test samples were labeled with Cy5 and hybridized to pooled control samples labeled with Cy3 from the same time-points. Competitive hybridization to the arrays was followed by washing, scanning, image analysis, and data analysis. Selected genes were analyzed with RT-qPCR.
2011-02-03 | E-GEOD-26981 | biostudies-arrayexpress
Project description:Characterisation of the maternal yolk associated protein (LsYAP) and establishment of systemic RNA interference in the salmon louse (Lepeophtheirus salmonis) (Crustacea, Copepoda)
2009-04-21 | E-TABM-685 | biostudies-arrayexpress
Project description:This study investigates host-specific gene expression of the Pacific salmon lice, Lepeophtheirus salmonis oncorhynchii, while parasitizing a resistant host (Coho salmon), two susceptible hosts (Atlantic salmon, Sockeye salmon), and a population with-held hosts (starved), over 48 hrs.
2017-01-25 | GSE80220 | GEO
Project description:Gene expression profiling revealed rapid activation of immunity, both local and systemic, which however did not provide protection of fish against the parasite. Major changes of transcriptome responses wwere observed between days 5 and 10 Atlantic salmon was challenged with L. salmonis at the copepod stage. Skin, spleen, and head kidney were sampled from challenged and control fish at 1, 3, 5 dpi (corresponding to the copepod stage); 10 and 15 dpi (chalimus stage). A total of forty samples of spleen and skin form infected salmon (4 individuals from the 5 time points) were used for microarray analyses.. Test samples were labeled with Cy5 and hybridized to pooled control samples labeled with Cy3 from the same time-points. Competitive hybridization to the arrays was followed by washing, scanning, image analysis, and data analysis. Selected genes were analyzed with RT-qPCR.
2011-02-03 | E-GEOD-26984 | biostudies-arrayexpress
Project description:Caligid copepods, also called sea lice, are common ectoparasites of wild and farmed marine fish. The salmon louse Lepeophtheirus salmonis (KrM-xyer, 1837) has emerged as a serious problem for salmon farming in the Northern hemisphere. The annual cost of sea lice to the global salmon mariculture industry has been estimated at M-^@300 million, of which the majority accounts for the cost of chemically treating the farmed salmon. The treatments available for salmonids with sea lice infestation have been limited with a large scale reliance on single products and the use of antiparasitics with similar modes of action, which when used over a long period of time can enhance the selection pressure for reduced sensitivity. The aim of the present study was to identify transcripts whose expression correlated to emamectin benzoate (EMB) susceptibility, or those genes regulated in response to EMB exposure. Two L. salmonis laboratory strains, established from field isolates and differing in susceptibility to EMB were studied using a custom sea louse 15K oligonucleotide microarray and RT-qPCR. Adult male sea lice were sampled from both strains after 1 and 3 hours of aqueous exposure to 0.2 M-5g mL-1 emamectin benzoate, 0.01% PEG300 or sea water. Bioinformatic analysis identified that in the absence of drug treatment, a large number of genes were significantly down regulated in the louse strain hyposensitive to EMB. EMB exposure had marked effects on gene expression in the EMB susceptible strain, but caused little changes in EMB hyposensitive lice. We therefore suggest that transcriptional responses induced by EMB exposure may not be responsible for reduced susceptibility to this antiparasitic compound, but may involve genes that are constitutively expressed in EMB tolerant salmon louse strains.
2013-11-06 | E-MTAB-1478 | biostudies-arrayexpress
Project description:Caligid copepods, also called sea lice, are common ectoparasites of wild and farmed marine fish. The salmon louse Lepeophtheirus salmonis (KrM-xyer, 1837) has emerged as a serious problem for salmon farming in the Northern hemisphere. The annual cost of sea lice to the global salmon mariculture industry has been estimated at M-^@300 million, of which the majority accounts for the cost of chemically treating the farmed salmon. The treatments available for salmonids with sea lice infestation have been limited with a large scale reliance on single products and the use of antiparasitics with similar modes of action, which when used over a long period of time can enhance the selection pressure for reduced sensitivity. Two L. salmonis laboratory strains, established from field isolates and differing in susceptibility to emamectin benzoate (EMB) were studied using a custom sea louse 15K oligonucleotide microarray and RT-qPCR. The aim of the present study was to identify differential expression of transcripts between these two strains to identify potential constitutive gene expression changes associated with reduced susceptibility to EMB. Adult male salmon lice were sampled without exposure to antiparasitic agents for the purpose of studying gene expression from unchallenged individuals. In this study changes in expression of Glutamate-gated Chloride channel (GluCl) subunits, considered the major target site for avermectin (AVM) drugs in invertebrates, was not observed, but expression changes were seen for alternative ligand-gated ion channel (LGIC) subunits that form an ion channels shown to interact with AVMs in vertebrates, but which is not traditionally considered to be a target site for AVMs in invertebrates. We hypothesise that these LGIC subunits represent additional EMB target sites in salmon lice, and that the down-regulation of these channel subunits in this EMB-resistant strain is related to the resistance phenotype.
2013-11-06 | E-MTAB-1484 | biostudies-arrayexpress
Project description:This study investigates sex-biased gene expression between populations of Atlantic and Pacific salmon lice, Lepeophtheirus salmonis. Two Atlantic L. salmonis populations were previously used for an array study (GSE56024) while a third dataset using Pacific L. salmonis was novel. Using all three populations, a consensus-based, meta-analysis approach was used to identify sex-biased and sex-specific genes.
2016-06-01 | GSE73734 | GEO
Project description:This study investigates sex-biased gene expression between populations of Atlantic and Pacific salmon lice, Lepeophtheirus salmonis. Two Atlantic L. salmonis populations were previously used for an array study (GSE56024) while a third dataset using Pacific L. salmonis was novel. Using all three populations, a consensus-based, meta-analysis approach was used to identify sex-biased and sex-specific genes. Two separate experiments were conducted, one for Atlantic and one for Pacific salmon lice. As the Atlantic data has been previously published for other comparisons (GSE56024), only the Pacific data is uploaded here. Lice from three populations (2 in the Atlantic and 1 in the Pacific) were collected for in vitro bioassay analysis using emamectin benzoate. After 24hrs, lice were collected as per treatment protocol below. Males and females from all populations were compared separately before forming a consensus probe list of sex-biased genes concordantly expressed across all three populations. Please note that each raw data file contains three or four 'block' data and each block data correspond to individual sample raw data. Therefore, each raw data file contains raw data for 3-4 samples (as indicated in the description field).
2016-06-01 | E-GEOD-73734 | biostudies-arrayexpress
Project description:We have utilized a microarray containing 11,100 salmon louse genes to study the gene expression patterns in selected salmon louse tissues: brain (neuronal and gland enriched tissue), subcuticular tissue, gut, ovary, and testis of adult louse.
2012-12-01 | E-MTAB-1339 | biostudies-arrayexpress
Project description:The salmon louse is an ectoparasitic copepod that causes major economic losses in the aquaculture industry of Atlantic salmon. This host displays a high level of susceptibility to lice which can be accounted for by several factors including stress. In addition, the parasite itself acts as a potent stressor of the host, and outcomes of infection can depend on biotic and abiotic factors that stimulate production of cortisol. Consequently, examination of responses to infection with this parasite, in addition to stress hormone regulation in Atlantic salmon, is vital for better understanding of the host pathogen interaction. Atlantic salmon post smolts were exposed to stress hormone cortisol, lice and their combination. The transcriptomic effects of hormone treatment in salmon skin were significantly greater than lice-infection induced changes. Cortisol stimulated expression of genes involved in metabolism of steroids and amino acids, chaperones, responses to oxidative stress and eicosanoid metabolism and suppressed genes related to antigen presentation, B and T cells, antiviral and inflammatory responses. Cortisol and liceBoth treatments equally down-regulated a large panel of motor proteins that can be important for wound contraction. Cortisol also suppressed multiple genes involved in wound healing, parts of which were activated by the parasite. Down-regulation of collagens and other structural proteins was in parallel with the induction of proteinases that degrade extracellular matrix (MMP9 and MMP13). Cortisol reduced expression of genes encoding proteins involved in formation of various tissue structures, regulators of cell differentiation and growth factors. Atlantic salmon post smolts were organised into four experimental groups: lice + cortisol, lice + placebo, no lice + cortisol, no lice + placebo. Infection levels were equal in both treatments upon termination of the experiment. Gene expression changes in skin were assessed with 21 k oligonucleotide microarray and qPCR at the chalimus stage 18 days post infection at 9oC.
2012-02-25 | E-GEOD-36072 | biostudies-arrayexpress