NsaRS is a Cell-Envelope-Stress Sensing Two-Component System of Staphylococcus aureus

ABSTRACT: S. aureus possesses 16 two-component systems (TCS), two of which (GraRS & NsaRS) belong to the intramembrane-sensing histidine kinase (IM-HK) family, conserved within the firmicutes. NsaRS has recently been documented as being important for nisin-resistance in S. aureus. In this study we present a detailed characterization of NsaRS and reveal that, as with other IM-HK TCS, it responds to disruptions in cell-wall stability. Analysis using a lacZ reporter-gene fusion demonstrated that nsaRS expression is upregulated by a variety of cell-wall damaging antibiotics, including Phosphomycin, Ampicillin, Nisin and Penicillin G. Additionally; we reveal that NsaRS regulates a downstream transporter, NsaAB, during nisin-induced stress. NsaS mutants also display a 200-fold decreased ability to develop

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Vijayaraj Nagarajan 

PROVIDER: E-GEOD-27061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress