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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Affymetrix SNP array data for different passages of mesenchymal stem cells.

ABSTRACT: Cells in culture undergo replicative senescence and unequivocally stop proliferation after a limited number of cell divisions. In this study, we have expanded mesenchymal stem cells (MSC) from human adipose tissue and analyzed genetic and epigenetic sequels. The subpopulation of highly proliferative cells and the in vitro differentiation potential decayed already within early passages. Relevant chromosomal aberrations were not detected by karyotyping and SNP-microarrays. Comparison of early and late passage of five samples of mesenchymal stem cells from human adipose tissue.

ORGANISM(S): Homo sapiens

SUBMITTER: L Gan

PROVIDER: E-GEOD-27135 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks.

Schellenberg Anne A Lin Qiong Q Schüler Herdit H Koch Carmen M CM Joussen Sylvia S Denecke Bernd B Walenda Gudrun G Pallua Norbert N Suschek Christoph V CV Zenke Martin M Wagner Wolfga W

Aging 20110901 9

Cells in culture undergo replicative senescence. In this study, we analyzed functional, genetic and epigenetic sequels of long-term culture in human mesenchymal stem cells (MSC). Already within early passages the fibroblastoid colony-forming unit (CFU-f) frequency and the differentiation potential of MSC declined significantly. Relevant chromosomal aberrations were not detected by karyotyping and SNP-microarrays. Subsequently, we have compared DNA-methylation profiles with the Infinium HumanMeth ...[more]

PMID: 22025769

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2012-09-24 | E-GEOD-37067 | biostudies-arrayexpress

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Project description:Surface topography impacts on cell growth and differentiation, but it is not trivial to generate homogeneous surface structures and to define the specific morphological parameters of relevance. In this study, we have compared gene expression profiles of mesenchymal stem cells (MSCs) on nanostructured groove/ridge surfaces. Patterns were generated in polyimide using multi beam laser interference. These structures affected cell size and orientation of human MSCs. Furthermore, the nano-patterns with a periodicity of 650 nm increased differentiation towards osteogenic and adipogenic lineages. However, in absence of differentiation media the surface structures did neither induce differentiation, nor lineage-specific gene expression changes – as assessed by genome wide gene expression profiles with Affymetrix microarray technology. Our results demonstrate that grooves and ridges at a periodicity of 650 nm enhance the propensity of MSCs to differentiate towards adipogenic and/or osteogenic lineages – but they do not directly govern lineage-specific gene expression changes. 9 samples were hybridized to the GeneChip Human Gene 2.0 ST Array (Affymetrix). In comparison to the manuscript the donor IDs refer as follows: donor 1 = AT57; donor 2 = AT58; donor 3 = AT61.

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