Transcription profiling of human SK-MEL-2 melanoma cell line transfected with E2F-1 and doxorubicin or transfected with a LacZ control plus doxorubicin to investigate the molecular mechanisms of a combination of E2F-1 gene therapy and chemotherapy.

ABSTRACT: SK-MEL-2 cells treated with Ad-E2F-1 (MOI 2), Ad-E2F-1 (MOI 2)plus doxorubicin 0.1uM, or Ad-LacZ (MOI 2) plus doxorubicin 0.1uM. The combination of E2F-1 gene therapy and chemotherapy produces a synergistic effect on melanoma cell apoptosis. However, the molecular mechanisms have not been fully elucidated. The purpose of this study was to identify novel genes or pathways that may play key roles in apoptosis when E2F-1 gene therapy is combined with doxorubicin chemotherapy. MATERIALS AND METHODS: SK-MEL-2 melanoma cells were infected with Ad-E2F-1 alone, Ad-E2F-1 plus doxorubicin, or Ad-LacZ plus doxorubicin. After 16 hours of treatment, the total RNA was extracted from these cells and subjected to microarray analysis. Quantitative real-time PCR was performed to confirm the microarray data.

ORGANISM(S): Homo sapiens

SUBMITTER: Hongying Hao 

PROVIDER: E-GEOD-2715 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress