Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Leptosphaeria maculans gene expression during in vitro growth and primary oilseed rape leaf infection

ABSTRACT: The transcriptome of Leptosphaeria maculans was analyzed in mycelium and during oilseed rape (Brassica napus) leaf infection. The array probes were designed from gene models from the L. maculans whole genome annotation. One aim of this study was to verify the expression of the automatically annotated gene models in various conditions. Another goal was to monitor gene expression profiles during oilseed rape leaf infection and to highlight tissue-specific transcripts, e.g. in plant up-regulated transcripts, for further analyses. We performed 9 hybridizations (NimbleGen) with samples derived from mycelium and infected oilseed rape leaves. Samples from infected oilseed rape leaves were harvested 7 and 14 days post infection. Three replicates each. All samples were labeled with Cy3.

ORGANISM(S): Brassica napus

SUBMITTER: Isabelle Fudal 

PROVIDER: E-GEOD-27152 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effector diversification within compartments of the Leptosphaeria maculans genome affected by Repeat-Induced Point mutations.

Rouxel Thierry T   Grandaubert Jonathan J   Hane James K JK   Hoede Claire C   van de Wouw Angela P AP   Couloux Arnaud A   Dominguez Victoria V   Anthouard Véronique V   Bally Pascal P   Bourras Salim S   Cozijnsen Anton J AJ   Ciuffetti Lynda M LM   Degrave Alexandre A   Dilmaghani Azita A   Duret Laurent L   Fudal Isabelle I   Goodwin Stephen B SB   Gout Lilian L   Glaser Nicolas N   Linglin Juliette J   Kema Gert H J GH   Lapalu Nicolas N   Lawrence Christopher B CB   May Kim K   Meyer Michel M   Ollivier Bénédicte B   Poulain Julie J   Schoch Conrad L CL   Simon Adeline A   Spatafora Joseph W JW   Stachowiak Anna A   Turgeon B Gillian BG   Tyler Brett M BM   Vincent Delphine D   Weissenbach Jean J   Amselem Joëlle J   Quesneville Hadi H   Oliver Richard P RP   Wincker Patrick P   Balesdent Marie-Hélène MH   Howlett Barbara J BJ  

Nature communications 20110215

Fungi are of primary ecological, biotechnological and economic importance. Many fundamental biological processes that are shared by animals and fungi are studied in fungi due to their experimental tractability. Many fungi are pathogens or mutualists and are model systems to analyse effector genes and their mechanisms of diversification. In this study, we report the genome sequence of the phytopathogenic ascomycete Leptosphaeria maculans and characterize its repertoire of protein effectors. The L  ...[more]

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