The Regulatory Specificity of a Homeodomain Protein is Determined by Unique DNA-Binding Sequences

ABSTRACT: Homeodomain (HD) proteins comprise a large family of evolutionarily conserved transcription factors (TFs) having diverse developmental functions, yet they paradoxically recognize very similar DNA sequences. To investigate how HDs control cell-specific gene expression patterns, we determined the DNA binding specificities of a broad range of HDs critical for Drosophila embryonic mesoderm development. These studies revealed particular sequences that are bound by one HD and not by others. Such HD-preferred binding sites are overrepresented in the noncoding regions of genes that are regulated by the corresponding HD. Moreover, we show at single-cell resolution in intact embryos that the HD Slouch (Slou) controls myoblast gene expression through unique DNA sequences that are preferentially b

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Brian Busser 

PROVIDER: E-GEOD-27163 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress