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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Ciliary neurotrophic factor induces genes associated with inflammatory response and gliosis in the retina: A gene profiling study of flow-sorted, Muller (glial) cells

ABSTRACT: Our data suggest that CNTF remodels the transcription profile of Muller (glial) cells leading to induction of networks associated with transcription, cell cycle regulation and inflammatory response. CNTF also appears to function as an inducer of gliosis in the retina. These studies provide new insights into the biological functions of cytokines in the retina. Muller glial cells treated with CNTF for 1 and 3 days

ORGANISM(S): Mus musculus

SUBMITTER: Radu Cojocaru

PROVIDER: E-GEOD-27195 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ciliary neurotrophic factor induces genes associated with inflammation and gliosis in the retina: a gene profiling study of flow-sorted, Müller cells.

Xue Wei W Cojocaru Radu I RI Dudley V Joseph VJ Brooks Matthew M Swaroop Anand A Sarthy Vijay P VP

PloS one 20110526 5

<h4>Background</h4>Ciliary neurotrophic factor (CNTF), a member of the interleukin-6 cytokine family, has been implicated in the development, differentiation and survival of retinal neurons. The mechanisms of CNTF action as well as its cellular targets in the retina are poorly understood. It has been postulated that some of the biological effects of CNTF are mediated through its action via retinal glial cells; however, molecular changes in retinal glia induced by CNTF have not been elucidated. W ...[more]

PMID: 21637858

Similar Datasets

Ciliary neurotrophic factor induces genes associated with inflammatory response and gliosis in the retina: A gene profiling study of flow-sorted, Muller (glial) cells

Project description:Our data suggest that CNTF remodels the transcription profile of Müller (glial) cells leading to induction of networks associated with transcription, cell cycle regulation and inflammatory response. CNTF also appears to function as an inducer of gliosis in the retina. These studies provide new insights into the biological functions of cytokines in the retina.

2011-09-01 | GSE27195 | GEO

Gene expression changes under cyclic mechanical stretch in rat retinal Muller cells

Project description:The retina is often subjected to tractional forces in a variety of conditions, for instance, pathological myopia, proliferative vitreoretinopathy. As the predominant glial element in the sensory retina, Muller cells are responsible for the homeostatic and metabolic support of retinal neurons and active players in virtually all forms of retinal injury and disease. Besides, Muller cells span the entire retinal thickness, extending from the inner to the outer limiting membranes, with cell bodies located in the inner nuclear layer and lateral processes expanding into the plexiform layers of the tissue. Because of this unique morphology, Muller cells can sense even minute changes in the retinal structure because of the mechanical stretching of their long processes or side branches. Thus, it’s reasonable to infer that Muller cells also participate in ocular diseases when the retina is overstretched. In this study, we aim to investigate the whole genome regulation of Muller cells under mechanical stretching, which may help in excluding possible molecular mechanisms that would account for many retinal diseases in which the retina is often subjected to mechanical forces. We used microarrays to identify patterns of gene expression changes induced by cyclic mechanical stretching in Muller cells.

2013-01-16 | GSE43516 | GEO

Gene expression changes under cyclic mechanical stretch in rat retinal Muller cells

Project description:The retina is often subjected to tractional forces in a variety of conditions, for instance, pathological myopia, proliferative vitreoretinopathy. As the predominant glial element in the sensory retina, Muller cells are responsible for the homeostatic and metabolic support of retinal neurons and active players in virtually all forms of retinal injury and disease. Besides, Muller cells span the entire retinal thickness, extending from the inner to the outer limiting membranes, with cell bodies located in the inner nuclear layer and lateral processes expanding into the plexiform layers of the tissue. Because of this unique morphology, Muller cells can sense even minute changes in the retinal structure because of the mechanical stretching of their long processes or side branches. Thus, itM-bM-^@M-^Ys reasonable to infer that Muller cells also participate in ocular diseases when the retina is overstretched. In this study, we aim to investigate the whole genome regulation of Muller cells under mechanical stretching, which may help in excluding possible molecular mechanisms that would account for many retinal diseases in which the retina is often subjected to mechanical forces. We used microarrays to identify patterns of gene expression changes induced by cyclic mechanical stretching in Muller cells. Rat Muller cells were seeded onto flexible bottom culture plates and subjected to a cyclic stretching regimen of 15% equibiaxial stretching for 1 and 24 h.Muller cells cultured under the same conditions but with no applied mechanical strain were considered as the unstretched control. At each time points (1 and 24 h), three totally independent experiments (3 stretched samples and 3 control samples) were conducted. Muller cells were selected for RNA extraction and hybridization on Affymetrix microarrays. Stretch (S); Control (C)

2013-01-16 | E-GEOD-43516 | biostudies-arrayexpress

DAPT-dependent regulation of zebrafish Muller glial cell chromatin accessibility

Project description:In this work we identify changes in Muller glial cell chromatin accessibility in response to DAPT-treatment

2021-07-31 | GSE160044 | GEO

Injury-dependent regulation of zebrafish Muller glial cell chromatin accessibility

Project description:In this work we identify changes in Muller glial cell chromatin accessibility in response to retinal injury

2021-07-31 | GSE160019 | GEO

Gene expression profile at single cell level of Wild type and Plagl1 knock out retina at P7

Project description:Plagl1 controls Muller glial cells proliferation in the retina. We used single cell RNA sequencing (scRNA-seq) to analyze the prolirative state of Muller glial cells and identify the signals that might be involved in this process

2024-03-24 | GSE228191 | GEO

DAPT-dependent regulation of zebrafish Muller glial cell gene expression

Project description:In this work we identify genes differentially expressed in Muller glia from uninjured retina in zebrafish treated with and without DAPT for 1 day.

2021-07-31 | GSE160047 | GEO

Injury and apobec2-dependent regulation of zebrafish Muller glial cell gene expression

Project description:In this work we identify genes differentially expressed in Muller glia form uninjured and injured retina with and without apobec2a knockdown

2020-05-19 | GSE145330 | GEO

DN-MAML-dependent regulation of zebrafish Muller glial cell gene expression

Project description:In this work we identify genes differentially expressed in Muller glia from uninjured retina in zebrafish treated with and without DN-MAML for 1 day.

2021-07-31 | GSE160051 | GEO

Impacts of ciliary neurotrophic factor on the retinal transcriptome in a mouse model of photoreceptor degeneration

Project description:Ciliary neurotrophic factor (CNTF) has been tested in clinical trials for human retinal degeneration due to its potent neuroprotective effects in various animal models. To decipher CNTF-triggered molecular events in the degenerating retina, high-throughput RNA sequencing analyses were performed using the Rds/Prph2 (P216L) transgenic mouse as a preclinical model for retinitis pigmentosa. Retinal transcriptome data was obtained for untreated wild type retinas, Rds retinas treated with a lentivirus expressing CNTF from postnatal day 25 to 35, and Rds retinas similarly treated with a control lentivirus expressing GFP. Comparisons of transcriptome data detect significant changes between wild type and Rds retinas. In addition, RNA-seq data reveal elevation of transcripts in the innate immune system and growth factor signaling, as well as altered neuronal transmission and metabolism in CNTF-treated Rds retinas. These results demonstrate the influence of exogenous CNTF on the retinal transcriptome landscape and illuminate likely CNTF impacts in degenerating human retinas.

2022-10-25 | GSE216208 | GEO

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