FACS-array profiling in retinal endothelial cells from living mouse retinas

ABSTRACT: Deregulated retinal angiogenesis directly cause vision loss in many ocular diseases, such as diabetic retinopathy and retinopathy of prematurity. To identify endothelial-specific genes expressed in angiogenic retinal vessels, we purified genetically labeled endothelial cells from Tie2-GFP transgenic mice and performed gene expression profiling using DNA microarray. To find out genes associated with angiogenesis, comparisons of microarray data were carried out between GFP-negative non-endothelial retinal cells and GFP-positive retinal endothelial cells in angiogenic P8 retina. Eighteen arrays are included. Utilizing fluorescence-activated cell sorting (FACS), we isolated endothelial cells as GFP-positive cells from P8 retina in homozygous Tie2-GFP transgenic mice. GFP-negative cells were se

ORGANISM(S): Mus musculus

SUBMITTER: Sentaro Kusuhara 

PROVIDER: E-GEOD-27238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress