Molecular Characterization of In Vivo Adjuvant Activity in Ferrets Vaccinated against Influenza Virus

ABSTRACT: The 2009 H1N1 influenza pandemic has prompted a significant need for the development of efficient, single-dose, adjuvanted vaccines. Here we investigated the adjuvant potential of CpG oligodeoxynucleotide (ODN) when used with a human seasonal influenza virus vaccine in ferrets. We found that the CpG ODNadjuvanted vaccine effectively increased antibody production and activated type I interferon (IFN) responses compared to vaccine alone. Based on these findings, pegylated IFN- 2b (PEG-IFN) was also evaluated as an adjuvant in comparison to CpG ODN and complete FreundM-bM-^@M-^Ys adjuvant (CFA). Our results showed that all three vaccines with adjuvant added prevented seasonal human A/Brisbane/59/2007 (H1N1) virus replication more effectively than did vaccine alone. Gene expression profiles in

ORGANISM(S): Mustela putorius furo

SUBMITTER: Longsi Ran 

PROVIDER: E-GEOD-27248 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress