Transcription profiling of human PBMCs from children infected with rotavirus in acute and convalescent phase reveals rotavirus infection alters peripheral T-cell homeostasis in children with acute diarrhea

ABSTRACT: Rotavirus infection is the single most important cause of severe diarrhea in young children worldwide. We used Affymetrix Human U95Av2 high density oligonucleotide arrays to compare gene expression profiles in peripheral blood mononuclear cells (PBMC) of 10 children with acute rotavirus diarrhea and 8 age-matched healthy children. We also examined patterns of gene expression in 5 convalescent-phase PBMC samples from rotavirus patients. For data analysis, we imported .cel files generated by Affymetrix MAS5.0 into Genetraffic 3.1 software (Iobion) and performed robust multi-chip analysis. We considered a gene in patients differentially expressed if its level of expression was at least 1.5-fold higher or lower than the baseline (arithmetic mean) of the corresponding gene in 8 controls and if

ORGANISM(S): Homo sapiens

SUBMITTER: Yu_Huan Wang 

PROVIDER: E-GEOD-2729 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress