Transcriptome partitioning in mouse fertilization.

ABSTRACT: RNA transcripts are distributed non-uniformly in the oocytes of many animals, such that newly-divided embryo cells (blastomeres) inherit distinct transcriptomes following fertilization. In animals such as the frog, Xenopus laevis, programmed transcript regionalization directs early embryonic axis formation and is essential for normal development. However, it is unknown whether such transcriptome asymmetry directs embryogenesis in mammals, or indeed, whether it occurs at all. We here address this by transcript profiling of matching sub-cellular structures and single cells in mouse oocytes and early embryos and analytical strategies exploiting the paired data structure. Spindle samples contained a set of transcripts that was distinguishable from that of the unfertilized metaphase II (mII) oo

ORGANISM(S): Mus musculus

SUBMITTER: Matthias Maneck 

PROVIDER: E-GEOD-27396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress