Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

76 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional response of an azole-resistant Candida parapsilosis isolate [voriconazole].

ABSTRACT: Azole resistance was induced in vitro by growth of a susceptible C. parapsilosis isolate in the presence of voriconazole. Whole genome microarrays were used to compare the transcriptional response of the voriconizole-resistant and susceptible isolates. Transcriptional profile of an in vitro derived voriconazole-resistant isolate of C. parapsilosis (BC014VRC) compared to the susceptible isolate (BC014S). Cell were grown in YPD medium in normoxia at 35 degrees. Each strain was labelled with Cy3 or Cy5. Overall, 4 independent biological replicates were compared; 2 dye swaps were performed to normalize dye effects.

ORGANISM(S): Candida parapsilosis

SUBMITTER: Geraldine Butler

PROVIDER: E-GEOD-27408 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcriptional response of an azole-resistant Candida parapsilosis isolate [posaconazole].

Project description:Azole resistance was induced in vitro by growth of a susceptible C. parapsilosis isolate in the presence of posaconazole. Whole genome microarrays were used to compare the transcriptional response of the posaconazole-resistant and susceptible isolates. Transcriptional profile of an in vitro derived posaconazole-resistant isolate of C. parapsilosis (BC014PSC) compared to the susceptible isolate (BC014S). Cell were grown in YPD medium in normoxia at 35 degrees. Each strain was labelled with Cy3 or Cy5. Overall, 3 independent biological replicates were compared; 1 dye swap was performed to normalize dye effects.

2011-04-18 | E-GEOD-27407 | biostudies-arrayexpress

Transcriptional response of an azole-resistant Candida parapsilosis isolate [fluconazole].

Project description:Azole resistance was induced in vitro by growth of a susceptible C. parapsilosis isolate in the presence of fluconazole. Whole genome microarrays were used to compare the transcriptional response of the fluconazole-resistant and susceptible isolates. Transcriptional profile of in vitro derived fluconazole resistant isolate of C. parapsilosis (BC014FLC) compared to susceptible isolate (BC014S). Cell were grown in YPD medium in normoxia at 35 degrees. Each strain was labelled with Cy3 or Cy5. Overall, 4 independent biological replicates were compared; 2 dye swaps were performed to normalize dye effects.

2011-04-18 | E-GEOD-27405 | biostudies-arrayexpress

Transcriptional response of an azole-resistant Candida parapsilosis isolate.

Project description:This SuperSeries is composed of the following subset Series: GSE27405: Transcriptional response of an azole-resistant Candida parapsilosis isolate [fluconazole]. GSE27407: Transcriptional response of an azole-resistant Candida parapsilosis isolate [posaconazole]. GSE27408: Transcriptional response of an azole-resistant Candida parapsilosis isolate [voriconazole]. Refer to individual Series

2011-04-18 | E-GEOD-27409 | biostudies-arrayexpress

Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans

Project description:This SuperSeries is composed of the following subset Series: GSE33460: Transcriptional profile of Candida albicans bcr1 knockout. GSE33461: Transcriptional profile of Candida parapsilosis bcr1 knockout. GSE33462: Transcriptional profile of Candida parapsilosis CLIB214 culture in low iron conditions Refer to individual Series

2011-11-05 | E-GEOD-33490 | biostudies-arrayexpress

Transcriptional profile of Candida parapsilosis bcr1 knockout.

Project description:Whole genome microarrays were used to compare the transcriptional profile of Candida parapsilosis bcr1 knockout to wild type cells. RNA was isolated from CLIB214 (wild type) or bcr1 delete (CDb71 or CDUHB6) grown in SD medium supplemented with 50 mM glucose and 10% fetal calf serum and labeled with Cy3 or Cy5. 7 independent biological replicates were compared. Two dye swaps were perfomed so that five of seven CLIB214 cultures were labeled with Cy3, and two were labeled with Cy5.

2011-11-04 | E-GEOD-33461 | biostudies-arrayexpress

Transcriptional profile of Candida parapsilosis CLIB214 culture in low iron conditions

Project description:Whole genome microarrays were used to generate the transcriptional profile of Candida parapsilosis in low iron conditions RNA was isolated from CLIB214 grown in SD medium and in SD medium supplemented with 200 μM BPS and labeled with Cy3 or Cy5. Seven independent biological replicates were compared. Three dye swaps were perfomed so that four out of seven samples grown in SD culture were labeled with Cy3, and 3 were labeled with Cy5.

2011-11-04 | E-GEOD-33462 | biostudies-arrayexpress

Hypoxia and biofilms in Candida parapsilosis

Project description:This SuperSeries is composed of the following subset Series: GSE13717: Transcriptional profile of Candida parapsilosis in SD media GSE13722: Transcriptional response of Candida parapsilosis in low oxygen (hypoxic) conditions in SD media Refer to individual Series

2010-05-06 | E-GEOD-13832 | biostudies-arrayexpress

Transcriptional response of Candida parapsilosis in low oxygen (hypoxic) conditions in SD media

Project description:Whole genome microarray was used to compare the transcriptional profile of C. parapsilosis growing in normoxic (21% oxygen) and hypoxic (1% oxygen) conditions. RNA was isolated from cells grown in SD media at 37Â°C for 3 h in atmosphere oxygen of 1% oxygen, and labeled with Cy5 or Cy3. Six independent biological replicates were compared. 4 out of 6 hypoxic samples were labeled with Cy5, and 2 were labeled with Cy3.

2010-05-06 | E-GEOD-13722 | biostudies-arrayexpress

Transcriptional response of an azole-resistant Candida parapsilosis isolate [voriconazole].

Project description:Azole resistance was induced in vitro by growth of a susceptible C. parapsilosis isolate in the presence of voriconazole. Whole genome microarrays were used to compare the transcriptional response of the voriconizole-resistant and susceptible isolates.

2011-04-18 | GSE27408 | GEO

Transcriptional response of an azole-resistant Candida parapsilosis isolate [posaconazole].

2011-04-18 | GSE27407 | GEO