Complex modulation of androgen responsive gene expression by methoxyacetic acid

ABSTRACT: Optimal androgen signaling is critical for testicular development and spermatogenesis. Methoxyacetic acid (MAA), the primary active metabolite of the industrial chemical ethylene glycol monomethyl ether, disrupts spermatogenesis and causes testicular atrophy. Transcriptional trans-activation studies have indicated that MAA can enhance androgen receptor activity, however, whether MAA actually impacts the expression of androgen-responsive genes in vivo, and which genes might be affected is not known. A mouse TM3 Leydig cell line that stably expresses androgen receptor (TM3-AR) was prepared and analyzed by transcriptional profiling to identify target gene interactions between MAA and testosterone on a global scale. MAA is shown to have widespread effects on androgen-responsive genes, affe

ORGANISM(S): Mus musculus

SUBMITTER: David Waxman 

PROVIDER: E-GEOD-27410 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress