Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

8 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Response of rice seedlings to the allelochemical juglone

ABSTRACT: Juglone is phytotoxic and has been widely studied for allelopathic effects, but the molecular mode of action of allelochemicals is not well understood.To gain more insight into cellular responses to juglone, we have taken a large-scale analysis of the rice transcriptome during juglone stress. Exposure to juglone triggered changes in the transcript levels for several genes related to cell rescue and defense, with the majority of these genes potentially associated with chemical detoxification pathways, abiotic stress response pathway, cell wall formation and epigenetic mechanism. Molecular mechanisms for the allelochemical,juglone, in rice roots Comparison of mock control and rice seedlings treated with 10mM juglone; Biological replicates: 3 control replicates, 3 juglone-treated replicates.

ORGANISM(S): Oryza sativa

SUBMITTER: Wen-Chang Chi

PROVIDER: E-GEOD-27413 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome and signalling response to As stress

Project description:Arsenic (As) is considered as the most common toxic metalloid, but its molecular mode of action is not well understood. In this study, we demonstrated that As(V) induced intracellular ROS production and Ca2+ accumulation in rice roots. To understand the molecular basis of plant cell responses to As better, we have undertaken a large-scale analysis of the rice transcriptome during As(V) stress. Up-regulated genes were enriched in transcripts involved in abiotic stress, chemical detoxification pathways, secondary metabolic process, transcription regulation and protein degradation while down-regulated genes were enriched in transcripts involved in secondary cell wall biogenesis, cell cycle and oligopeptide transporter. Molecular mechanisms for the As(V) in rice roots Comparison of mock control and rice seedlings treated with 25 M-NM-<M As(V); Biological replicates: 3 control replicates, 3 As(V)-treated replicates.

2012-10-21 | E-GEOD-33375 | biostudies-arrayexpress

HepG2 Cell: Control vs. HGF treated

Project description:Transcription profile of HepG2 cells treated with hepatocyte growth factor and control cells Two condition experiment, Hep G2 vs. Hep G2-HGF. Biological replicates: 1 control and 1 HGF-treated (no replication)

2012-02-23 | E-GEOD-35994 | biostudies-arrayexpress

Autotoxicity mechanism of Oryza Sativa: Microarray analysis of rice plants in response to allelochemical ferulic acid

Project description:Autotoxicity plays an important mechanism in regulating plant productivity. Ferulic acid (FA) is phytotoxic and was identified in extracts and residues of rice plants as a candidate for rice allelochemicals. To help characterize the autotoxicity mechanism of rice, we present the first large-scale, transcriptomic analysis of rice root responses to ferulic acid. Two-condition experiment, short exposures and long exposures. Comparison of mock control and rice seedlings treated with 50 ppm ferulic acid (FA) during short (pooled from 1- and 3-h treatments), as compared to long (24 h) exposures.; Biological replicates: 3 control replicates (short and long exposures), 3 FA-treated replicates (short and long exposures).

2013-07-22 | E-GEOD-34899 | biostudies-arrayexpress

Comparison of the gene expression profiles between iPS-OSH, iPS-J, ESC and MEF.

Project description:iPS-OSH was the novel iPS cells generated from our lab, the microarray analysis was used for determining the gene expression profiles of iPS-OSH. iPS-J was provided from Dr. Yamanaka (Riken, APS0001). Microarray analysis was used to compare the difference of gene expression profiles in iPS-OSH, iPS-J, ESC and MEF. The total RNA extracted from iPS-OSH, iPS-J, ESC and MEF was used for the microarray analysis to compare the gene expression profiles.

2012-07-31 | E-GEOD-31822 | biostudies-arrayexpress

Giardia lamblia WB: 5'5npac control vs. pPPax2

Project description:Transcriptional profiling of Giardia lamblia WB comparing control 5'5npac cells with pPPax2 cells. Two-condition experiment 5'5npac cells with pPPax2 cells.

2012-12-01 | E-GEOD-30875 | biostudies-arrayexpress

Mesenchymal progenitors from hESCs & profiling vs. BMMSCs

Project description:New potential sources of stem cells for clinical application include bone marrow mesenchymal stem cells (BMMSCs), human embryonic stem cells (hESCs), and induced pluripotent stem cells (iPS). However, each source is not without its own concerns. While research continues in an effort overcome these problems, the generation of mesenchymal progenitors from existing hESC lines may circumvent many of these issues. We report here a comparison of the transcriptome of hESC-derived mesenchymal progenitors (EMPs), its parental hESC line, and 2 donors of adult BMMSCs. A custom-designed oligo-microarray of just over 11,000 highly-expressed genes in stem cells was used to profile the transcriptome of BMMSCs, EMPs, and hESCs.

2012-01-01 | E-GEOD-18543 | biostudies-arrayexpress

Chromium stress response in rice roots: effects on transcriptome proﬁles and signalling pathways responsive

Project description:Chromium (Cr) is an important environmental contaminant and also a genotoxic metal ion at high concentration, but its molecular mechanisms and signalling pathways of action in plants is still poorly unraveled. To help characterize the mechanism of molecular and signalling of rice, we present the large-scale, transcriptomic analysis of rice root responses to Cr(VI). We examined the possible involvement of reactive of reactive oxygen species (ROS) and calcium in Cr(VI) signaling transduction pathways, as well as the effect of Cr (VI) on CDPK and MAPK activity. Specially, we used the microarray assay to assess different stage Cr (VI) induced alteration in rice gene expression. This gene discovery effort will help expand our understanding of cellular responses to Cr (VI) treatment, and will identify candidate genes for enhancement of Cr resistance in crop. Two-condition experiment, short exposures and long exposures. Comparison of mock control and rice seedlings treated with 50 μM Cr(VI) during short (pooled from 1- and 3-h treatments), as compared to long (24 h) exposures.; Biological replicates: 3 control replicates (short and long exposures), 3 Cr(VI)-treated replicates (short and long exposures).

2014-12-21 | E-GEOD-41733 | biostudies-arrayexpress

Human gastric cancer cell MKN45: MKN45-GFP control vs. highly metastatic sublines

Project description:Gene expression profiles of in vitro selected highly metastatic MKN45-GFP sublines. The results were compared with MKN45-GFP control cell line to determine the metastasis associated genes. Four pairs compared experiment. Each pair was used MKN45-GFP cells as correlated control. Determining on the gene expression trends were by various metastatic ability of each subline.

2011-11-10 | E-GEOD-33570 | biostudies-arrayexpress

Transcription profiling of human trisomy 21 amniocytes and euploid amniocytes

Project description:Congenital development disorders with variable severity occur in trisomy 21. However, how these phenotypic abnormalities develop with variations remains elusive. We hypothesize that the difference in euploidy gene expression variation among trisomy 21 tissues are perturbed by the presence of an extra copy of chromosome 21 and this may contribute to the phenotypic variations in Down syndrome. Experiment Overall Design: We used DNA microarray to measure the differences in gene expression variance (representing variation) between four human trisomy 21 amniocytes and six human euploid amniocytes.

2008-04-03 | E-GEOD-10758 | biostudies-arrayexpress

The transcriptome of anthers of rice gamyb mutant expressing various GAMYB homologs was measured at the microspore stage.

Project description:To confirm the function of the GAMYB homologs for Selaginella moellendorffii and Physcomitrella patens in rice cells, we examined the expression profile in the anthers of transgenic plants, which OsGAMYB, SmGAMYB, or PpGAMYB2 is expressed under the control of the rice GAMYB promoter in rice gamy mutant. Expression profiling was conducted using rice 4x44K microarray RAP-DB (G2519F#15241). The microarray was used to profile gene expression in OsGAMYB:OsGAMYB, OsGAMYB:SmGAMYB, OsGAMYB:PpGAMYB2 and vector transgenic plants. To assess the reproducibility of the microarray analysis, each microarray experiment was performed twice using RNA samples that had been isolated independently (i.e., two biological replicates), and included a dye-swapping test as a technical replicate for each biological replicate.

2011-11-07 | E-GEOD-32652 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data