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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human HT29 colorectal adenocarcinoma cell line treated with apratoxin A vs. controls to determine the mode of action of apratoxin A

ABSTRACT: Total RNA was extracted from apratoxin A or vehicle treated HT29 cells using the RNeasy Mini Kit (Qiagen). Probe values from CEL files were condensed to probe sets using Rosetta Resolver software. Resolver ANOVA analysis was then performed between groups. Experiment Overall Design: 2 doses were compared to vehicle at 3 time points. Each time point had its own vehicle control

ORGANISM(S): Homo sapiens

SUBMITTER: John Walker

PROVIDER: E-GEOD-2742 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A functional genomics approach to the mode of action of apratoxin A.

Luesch Hendrik H Chanda Sumit K SK Raya R Marina RM DeJesus Paul D PD Orth Anthony P AP Walker John R JR Izpisúa Belmonte Juan Carlos JC Schultz Peter G PG

Nature chemical biology 20060212 3

The cyanobacterial metabolite apratoxin A (1) demonstrates potent cytotoxicity against tumor cell lines by a hitherto unknown mechanism. We have used functional genomics to elucidate the molecular basis for this activity. Gene expression profiling and DNA content analysis showed that apratoxin A induces G1-phase cell cycle arrest and apoptosis. Cell-based functional assays with a genome-wide collection of expression cDNAs showed that ectopic induction of fibroblast growth factor receptor (FGFR) ...[more]

PMID: 16474387

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